Hybridoma: Leading scientist has extensive experience, previously worked for Novartis Shanghai and Wuxi Biologics, Shanghai. Successfully developed x therapeutic antibody candidates covers wide variety of diseases and targets include (membrane protein, serum protein, etc), familiar with various kinds of techniques involved in DNA immunization.
Phage Display: Leading scientists have extensive experience in phage ScFv library construction, phage panning and characterization. Unique experience on panning internalization antibody with living target cells.
Computer Aided Design: Schordinger Bioluminate and Maestro. Ab Studio has humanized and optimized more than 20 antibodies from Mar 2017 to Oct 2017.
A “Knob-into-hole plus common LC” version allowing two arms to have very different binding affinity to each one’s own antigen.
Imbalanced binding affinity is required for the follow three applications:
If VLa and VLb light chain homology >80%, design a VLc based on VLa via computer aided design: Maintain VHaVLc’s affinity as close as that of VHaVLa, allow VHbVLc to have reduced affinity compared to VHbVLb.
If VLa and VLb light chain homology < 80%, construct phage ScFV library: VHs of the ScFV library are human germline VH library VLs of the ScFV library are VLa and its mutants derived from error prone PCR at less than 20% of mutation frequency.
Use computer aided design to separate the biochemical and biophysical features of VHa and VHb in order to isolate AB from AA and BB at a higher efficiency.